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Volume 2018 |Article ID 9105261 |

Wanda C. Reygaert, "Green Tea Catechins: Their Use in Treating and Preventing Infectious Diseases", BioMed Research International, vol. 2018, Article ID 9105261, 9 pages, 2018.

Green Tea Catechins: Their Use in Treating and Preventing Infectious Diseases

Academic Editor: Chedly Chouchani
Received20 Mar 2018
Accepted10 Jun 2018
Published17 Jul 2018


Green tea is one of the most popular drinks consumed worldwide. Produced mainly in Asian countries from the leaves of the Camellia sinensis plant, the potential health benefits have been widely studied. Recently, researchers have studied the ability of green tea to eradicate infectious agents and the ability to actually prevent infections. The important components in green tea that show antimicrobial properties are the catechins. The four main catechins that occur in green tea are (-)-epicatechin (EC), (-)-epicatechin-3-gallate (ECG), (-)-epigallocatechin (EGC), and (-)-epigallocatechin-3-gallate (EGCG). Of these catechins, EGCG and EGC are found in the highest amounts in green tea and have been the subject of most of the studies. These catechins have been shown to demonstrate a variety of antimicrobial properties, both to organisms affected and in mechanisms used. Consumption of green tea has been shown to distribute these compounds and/or their metabolites throughout the body, which allows for not only the possibility of treatment of infections but also the prevention of infections.

1. Introduction

Infectious diseases are a leading cause of morbidity and mortality worldwide. HIV/AIDS and malaria are among the top ten infectious diseases in the world; and the most common types of infections are respiratory tract and diarrheal diseases [1]. With the advent of antimicrobial agents in the mid-1900s came the hope that eradication of infectious diseases was close. Unfortunately, the microorganisms involved were able to become resistant to the antimicrobial agents, and that only made it harder to fight these organisms. The CDC has estimated that each year more than two million people in the US suffer from antibiotic-resistant infections and that as many as 23,000 people die each year from these infections [2]. This results in not only increased morbidity and mortality, but also increased healthcare costs, which can be a huge financial burden for many countries. A recent analysis of the medical costs from healthcare-associated infections (those infections acquired in a healthcare facility) alone estimated that the annual costs of these infections in the US are between 28 and 45 billion dollars [3]. Antimicrobial resistance issues continue to impact these costs. One study found that the cost of antimicrobial resistance associated illnesses in the US could be as high as 55 billion dollars (20 billion dollars for healthcare costs and 35 billion dollars for lost productivity) annually [4]. To help in the fight against infectious diseases, researchers are looking at the possibilities of using natural plant products, which could turn out to provide a tremendous cost savings in healthcare. One of the plants that is currently being widely studied is the tea plant, looking especially at green tea.

Tea is one of the most commonly consumed beverages in the world, and green tea is becoming increasingly popular, accounting for around 20% of total global tea production. Tea is produced from the Camellia sinensis plant and is grown in over 30 countries. The best areas for growing tea plants are in specific tropical and subtropical regions. There are four main tea types produced: white, green, Oolong, and black tea. The type of tea is determined by how the tea leaves are processed, specifically by drying and fermentation methods. White tea is processed the least and uses very young leaves and leaf buds. Green tea is produced from more mature leaves with no fermentation. Oolong tea is produced by partially fermenting the leaves and black tea by fully fermenting the leaves [57]. Green tea is most commonly consumed in China, Japan, and Korea. Black tea is most commonly consumed in the US and the UK [8].

Green tea has been shown to have anticarcinogenic, anti-inflammatory, antimicrobial, and antioxidant properties and is beneficial in cardiovascular disease (CVD), diabetes and obesity, and neurologic and oral health. The anticarcinogenic properties include controlling cell proliferation, apoptosis and angiogenesis in tumor cells [912]. Inflammation is a component of many conditions and diseases including aging, arthritis, cancer, CVD, diabetes, and obesity. The general anti-inflammatory properties of green tea include the ability to decrease the denaturation of proteins and increase the production of anti-inflammatory cytokines [7, 13]. Oxidative stress results from the damaging effects of reactive oxygen species (ROS). The antioxidant properties of green tea include the ability to limit the amount of free radicals by binding to ROS, upregulating basal levels of antioxidant enzymes, and increasing the activity of these antioxidant enzymes [6, 14, 15]. The effects of green tea on CVD include the anti-inflammatory and antioxidant effects. In addition, the consumption of green tea has been shown to inhibit atherosclerosis, reduce total lipid levels, and improve the ratio of LDL to HDL [16, 17]. Diabetes and obesity are closely associated with a spectrum of disorders known as metabolic syndrome (MetS) which includes increased waist diameter, elevated plasma triglycerides, decreased HDL, increased fasting blood glucose, and elevated blood pressure [18, 19]. Type 2 diabetes is also associated with insulin resistance and sometimes decreased insulin production. Green tea has been shown to increase insulin receptor sensitivity and stimulate glucose-induced insulin secretion [20, 21]. Obesity is a result of an increase in fat mass which is caused by increase in the size of fat cells. Green tea has been shown to inhibit digestive enzymes and absorption of fat, which leads to decreased body waist circumference, intra-abdominal fat, plasma total and LDL cholesterol, triglycerides, and blood pressure [2224]. The challenges of inflammation and oxidative stress can lead to DNA damage, protein misfolding, and loss of ATP production in mitochondria. This can result in cell death and loss of cognitive functions in the brain. The anti-inflammatory and antioxidant properties of green tea also protect neurons, and green tea metabolites have been shown to cross the blood brain barrier [2529]. Green tea has been shown to be antimicrobial against most oral bacteria. In addition, it has been shown to improve oral health by increasing the activity of oral peroxidases, preventing the development and progression of periodontitis, and reducing dentin erosion and tooth loss, and it has a role in improving bad breath [3034].

2. Green Tea Composition

The components in green tea that are the most medically relevant are the polyphenols. The most pertinent polyphenols are the flavonoids; and the most pertinent flavonoids are the catechins. The catechins comprise 80-90% of the flavonoids and around 40% of the water-soluble solids in green tea. Green tea contains more catechins than the other teas, mainly because of the way it is processed after harvesting. The amount of catechins in green tea can also be affected by where the tea is grown, the growth conditions, when it is harvested, how the leaves are processed, and the brewing temperature and length of time of brewing. These factors lead to a huge variation in catechin content among the varieties and brands of green tea consumed [3545].

The four main catechins found in green tea are (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin-3-gallate (ECG), and (-)-epigallocatechin-3-gallate (EGCG). The most abundant catechin is EGCG (~60%), and the next most abundant is EGC (~20%), then ECG (~14%), and EC (~6%). EGCG is the most studied in association with health, but EGC and ECG have been studied as well. As mentioned above, there can be a wide variation in the amount of catechins in any particular green tea beverage, although standardized extracts are available for use as supplements [7, 46, 47].

In order to be effective in the body these catechins need to be bioavailable after consumption. Once in the body, the catechins undergo metabolic processing in the liver and small intestine and colon. This processing produces glucuronide and sulfate conjugates or methyl epicatechins. Native forms of ECG and EGCG and metabolites of EC and EGC can be detected and measured in blood plasma. No forms of ECG and EGCG can be detected in urine, only metabolites of EC and EGC [48, 49]. Catechins are generally most stable in solution at a pH range of 4-6. It is now known that human serum albumin acts as a stabilizer, binding to the catechins and then transporting them [50]. Various studies in humans have found that the peak concentrations of catechins and their metabolites occur in blood plasma between 1.5 and 2 hours after ingestion and in urine between 4 and 6 hours after ingestion. The levels of these peak concentrations are affected by an individual’s metabolism and of course by the amount of catechins in the ingested type of green tea. Commonly, the levels found in the body are directly proportional to the amount of catechins consumed [5153]. Tables 1 and 2 show examples of blood plasma and urine concentration studies in humans.

EGCG dose (mg) (ng/ml) Reference

200 73.7 [54]
400 111.8
600 169.1
800 438.5

400 137.6 [55]
800 234.9

88 135 [56]
140 34.7 [57]

225 300 [58]
375 1970
525 2020

50 130.4 [59]
100 180.4
200 332.2
400 624.5
800 1067.4
1600 3391.6

200 376.9 [60]
400 525.2
800 1682.1

110 119 [61]
219 326
329 321

EGC dose (mg) Amount in 24 hour urine (mg) Reference

37 0.4 [54]
74 1.4
111 3.5
148 3.7

82 ~3.0 [56]
154 3.5 [57]
148 10.5 [62]

102 ~3.0 [61]
204 ~4.0
306 ~4.8

3. Antimicrobial Properties

The antimicrobial effects of green tea catechins (GTCs) on microorganisms have been studied for many years. Green tea has been shown to combat these organisms in various ways, directly and indirectly, and has been shown to work synergistically with some antibiotic agents. Other known health benefits of green tea such as the anti-inflammatory and antioxidant effects may also contribute to the antimicrobial effects. Studies conducted on Escherichia coli found that exposure to green tea polyphenols (GTPs) resulted in major gene expression changes for 17 genes, with upregulation occurring in nine genes and downregulation in eight genes [7577]. Table 3 shows a summary of the antimicrobial effects of green tea on bacteria.

Organism Effects References

Cell Membrane Binding to bacterial cell membrane [6365]
Associated Effects Damaging bacterial cell membrane [63]
Inhibits ability of bacteria to bind to host cells [66, 67]
Inhibits ability of bacteria to form biofilms [66, 68, 69]
Disrupts bacterial quorum sensing [68]
Interferes with bacterial membrane transporters [65, 69, 70]

Bacterial Cell Functions Inhibits bacterial DNA gyrase [71]
Effects Reduces bacterial H2S production [66]
Inhibits bacterial hemolytic action [66, 72]
Inhibition of bacterial DHFR enzyme [53]
Inhibits bacterial fatty acid synthesis enzymes [73]
Increases bacterial internal ROS levels [74]

4. Effects on the Bacteria Cell Membrane

One of the major properties of GTCs is the ability to bind to bacterial cell membranes. This binding can lead to interference in various bacterial processes and can damage the cell membrane resulting in increased permeability and leading to cell lysis. Because EGCG is negatively charged it can combine with the positively charged bacterial cell membrane, especially in gram positive bacteria. The lipopolysaccharide (LPS) on the outer membrane of gram negative bacteria makes them more resistant to binding by GTCs [53, 63, 64, 66]. Studies with E. coli and Pseudomonas aeruginosa have shown that EGCG binding to the bacterial cell membrane can result in generation of H2O2 which is involved in damage to the cell membrane [63, 74]. Studies with Staphylococcus aureus have shown that this assault on the cell membrane causes a major cell wall stress response, resulting in upregulation of peptidoglycan biosynthesis genes and an alteration in cell wall structure. In methicillin-resistant Staphylococcus aureus (MRSA) strains, this change in peptidoglycan biosynthesis genes results in the production of PBP2 (penicillin-binding protein 2), which is what confers resistance to β-lactam drugs. Production of PBP2 is also inhibited by EGCG [64, 78, 79]. An important result of green tea binding is the loss of bacterial ability to bind to host cells. Studies using human and mammalian cells lines have shown that various bacteria such as Fusobacterium nucleatum, Staphylococcus epidermidis, and Helicobacter pylori have significantly decreased adherence to these cells [66, 67, 80]. Other important results are the loss of the ability for quorum sensing and biofilm formation of P. aeruginosa, F. nucleatum, and Streptococcus mutans [66, 68, 81]. Damage to the cell membrane also results in loss of function to transmembrane transporter proteins which are responsible for secretion of toxins and efflux of substances such as antimicrobial agents [53, 65, 69, 70].

5. Effects on Other Bacterial Cell Functions

There are a wide variety of other effects that GTCs have on bacterial functions. An important one which can affect most bacteria is the ability of GTCs to inhibit bacterial fatty acid biosynthesis by inhibiting enzymes involved in the biosynthetic pathway. Because this is an essential pathway for most bacteria, researchers are looking at targeting this pathway in antimicrobial drug development. Fatty acids are important for building cell membranes, as an energy source, and are involved in the production of toxic bacterial metabolites [53, 73]. Another target is the folate biosynthesis pathway. The enzyme dihydrofolate reductase (DHFR) is essential in this pathway, and is known to be a target for certain sulfa drugs. EGCG has also been shown to inhibit DHFR activity [53, 82]. Other important effects against enzymes include inhibition of bacterial DNA gyrase, inhibition of bacterial ATP synthase activity, and inhibition of bacterial protein tyrosine phosphatase and cysteine proteases [53, 71, 83]. Some specific bacterial effects include reducing bacterial H2S production and inhibiting hemolytic activity of F. nucleatum, inhibiting the ability of Listeria monocytogenes to escape from the macrophage phagosome by inhibiting activity of listeriolysin O, and inhibiting the ability of E. coli to transfer plasmid content via conjugation [66, 72, 84].

6. Synergism

Since GTCs are known to have antimicrobial action, researchers have begun assessing the potential synergism of these catechins with other known antimicrobial agents. Green tea catechins have now been shown to act in synergy with imipenem against MRSA; with metronidazole against Porphyromonas gingivalis; with azithromycin, cefepime, ciprofloxacin, chloramphenicol, doxycycline, erythromycin, nalidixic acid, piperacillin, or tobramycin against E. coli; with ampicillin, Cefalotin, doxycycline, erythromycin, penicillin, or tetracycline against Enterobacter aerogenes; with chloramphenicol or tetracycline against Pseudomonas aeruginosa; and with aztreonam, ceftazidime, ciprofloxacin, gentamicin, meropenem, or tetracycline against Acinetobacter baumannii. The ability of GTCs to inhibit the function of bacterial efflux pumps (as mentioned previously) also plays a role in at least an additive antimicrobial effect for GTCs and many antimicrobial drugs, especially in gram negative bacteria that possess RND-type efflux pumps [53, 69, 70, 8589]. Table 4 lists antimicrobial agents that have shown synergy with GTCs and the targets of these drugs.

Antimicrobial Action Drug Synergism

Inhibit Cell Wall Synthesis ampicillin

Inhibit Protein Synthesis amikacin

Inhibit Nucleic Acid Synthesis ciprofloxacin
nalidixic acid

7. Effects on Other Microorganisms

Green tea catechins have also been shown to be effective against a number of viruses, parasites, fungi, and even prions. The main antiviral effects include inhibiting the virus from binding to and entering host cells (adenovirus, enterovirus, HBV, HCV, HIV, HSV, influenza, and rotavirus); inhibiting viral RNA and DNA synthesis and viral gene transcription (enterovirus, EBV, HBV, HCV, and HIV); and destroying and functionally altering various viral molecules (adenovirus, HSV, and influenza) [64, 9096]. Studies performed with adult healthcare workers to determine if green tea supplements could prevent infection with viruses causing influenza showed significantly fewer instances of influenza symptoms and a reduced incidence of laboratory-confirmed influenza cases versus the control group [97].

The main effect of GTCs on various parasite infections is a decrease in parasite numbers and growth. Other effects noted were fragmentation of parasite DNA and reduced fatty acid synthesis in the parasites. Studies with parasites include Plasmodium falciparum, Babesia spp., Trypanosoma brucei, Trypanosoma cruzi, and Leishmania braziliensis [98102].

Fungi that have been affected by GTCs include Aspergillus niger, Candida spp., Penicillium sp., Microsporum canis, Trichophyton mentagrophytes, and Trichophyton rubrum. Research testing for synergistic effects found that EGCG showed synergism with amphotericin B, fluconazole, and miconazole in Candida spp.; and in Candida tropicalis strains that were resistant to fluconazole, EGCG, and fluconazole together induced apoptosis in the yeast cells [64, 103107].

Prions are proteins that are considered to be infective agents because the abnormally structured (β-sheet) forms are able to induce normally structured (α-helix) forms to change shape. In the abnormal shape, protein function is lost and protein aggregation occurs in cells. Unlike other infectious agents, prions cannot be destroyed using autoclaving; the proteins have to be degraded to be noninfectious. Research using yeast cells found that EGCG could inhibit the β-sheet prions from changing the α-helical forms and could induce reversal of the β-sheet forms back to α-helical forms [108].

8. Antimicrobial Scope

There is a large amount of research that has assessed the antimicrobial effects of green tea catechins on a wide variety of microorganisms, including many gram negative and gram positive bacteria, some viruses, fungi, and prions. One of the most clinically important bacteria that has been researched is S. aureus, especially MRSA strains. The most studied gram negative bacteria is E. coli which is known for causing the majority of urinary tract infections. There are several recently published manuscripts that contain extensive information on which organisms are affected by green tea catechins [53, 64, 96, 109].

9. Prevention of Infection

Since it has been shown that GTCs have multiple types of antimicrobial abilities against so many organisms, it would be expected that green tea catechins could also prevent infections. One study was mentioned previously describing how green tea reduced the number of colds and influenza incidents. Another study involving adults showed that consuming green tea supplements twice daily for 3 months resulted in 32% fewer instances of cold or influenza symptoms and nearly 23% fewer illnesses of 2 or more days duration [110]. A study involving children found that, in school-aged children who consumed green tea on a regular basis, the number of incidents of influenza A or B was inversely associated with the number of cups of green tea consumed per day or per week [111]. Another study with Japanese nursey school children who gargled with green tea (or placebos) at least once each day found that there were up to 3 times fewer instances of illnesses with fevers in the green tea gargling group [112]. Two other studies with adults found that gargling with a green tea extract (GTE) solution resulted in at least half as many cases of influenza in the GTE gargling groups compared with the control groups [113, 114].

10. Conclusions

The research into the effects of green tea on human health has shown that it can be an important dietary factor in the prevention and treatment of various diseases such as arthritis, cancer, CVD, diabetes and obesity, infections, and in neurologic and oral health. Studies that were originally performed in animals and cell lines have become more frequently performed using humans. This type of research is vital if we are to fully discover what benefits GTCs can have in health issues. The more researchers that become involved in this, the clearer the answers. The studies on antimicrobial effects are providing very promising data, especially if GTCs prove to have synergistic abilities with many of the currently used antimicrobial agents and perhaps with drugs used to treat other diseases. The emergence of various multidrug-resistant bacteria, along with a dearth of effective antimicrobial drugs, makes the potential of green tea an extremely timely issue. There are also many areas across the globe where the cost of drugs is currently beyond the earning power of most of the population. Green tea is relatively inexpensive and fairly easy to obtain for most people. It could prove to be an answer for improving health on a global scale.

Conflicts of Interest

The author declares that there are no conflicts of interest regarding the publication of this paper.


  1. World Health Organization, “World Health Organization,” 2014. View at: Google Scholar
  2. U.S. Department of Health and Human Services Centers for Disease Control and Prevention. "Antibiotic resistance threats in the United States" 2013.
  3. P. W. Stone, “Economic burden of healthcare-associated infections: An American perspective,” Expert Review of Pharmacoeconomics & Outcomes Research, vol. 9, no. 5, pp. 417–422, 2009. View at: Publisher Site | Google Scholar
  4. R. Smith and J. Coast, “The true cost of antimicrobial resistance,” BMJ, vol. 346, no. 7899, Article ID f1493, 2013. View at: Publisher Site | Google Scholar
  5. D. Botten, G. Fugallo, F. Fraternali, and C. Molteni, “Structural Properties of Green Tea Catechins,” The Journal of Physical Chemistry B, vol. 119, no. 40, pp. 12860–12867, 2015. View at: Publisher Site | Google Scholar
  6. D. A. Gupta, D. J. Bhaskar, and R. K. Gupta, “Green tea: a review on its natural anti-oxidant therapy and cariostatic benefits,” Biological Sciences and Pharmaceutical Research, vol. 2, pp. 8–12, 2014. View at: Google Scholar
  7. A. Jigisha, R. Nishant, K. Navin et al., “Green tea: a magical herb with miraculous outcomes,” International Research Journal of Pharmacy, vol. 3, no. 5, pp. 139–148, 2012. View at: Google Scholar
  8. K. Hayat, H. Iqbal, U. Malik, U. Bilal, and S. Mushtaq, “Tea and its consumption: benefits and risks,” Critical Reviews in Food Science and Nutrition, vol. 55, no. 7, pp. 939–954, 2015. View at: Publisher Site | Google Scholar
  9. K. D. Crew, K. A. Ho, P. Brown et al., “Effects of a green tea extract, Polyphenon E, on systemic biomarkers of growth factor signalling in women with hormone receptor-negative breast cancer,” Journal of Human Nutrition and Dietetics, vol. 28, no. 3, pp. 272–282, 2015. View at: Publisher Site | Google Scholar
  10. M.-J. Li, Y.-C. Yin, J. Wang, and Y.-F. Jiang, “Green tea compounds in breast cancer prevention and treatment,” World Journal of Clinical Oncology, vol. 5, no. 3, pp. 520–528, 2014. View at: Publisher Site | Google Scholar
  11. Y. Shirakami, H. Sakai, T. Kochi, M. Seishima, and M. Shimizu, “Catechins and its role in chronic diseases,” Advances in Experimental Medicine and Biology, vol. 929, pp. 67–90, 2016. View at: Publisher Site | Google Scholar
  12. C. Subramani and R. K. Natesh, “Molecular mechanisms and biological implications of green tea polyphenol, (-)-epigallocatechin-3-gallate,” International Journal of Pharma Bioscience and Technology, vol. 1, no. 2, pp. 54–63, 2013. View at: Google Scholar
  13. P. Chaterjee, S. Chandra, P. Dey et al., “Evaluation of anti-inflammatory effects of green tea and black tea: a comparative in vitro study,” Journal of Advanced Pharmaceutical Technology & Research, vol. 3, no. 2, pp. 136–138, 2012. View at: Google Scholar
  14. B. J. Newsome, M. C. Petriello, S. G. Han et al., “Green tea diet decreases PCB 126-induced oxidative stress in mice by up-regulating antioxidant enzymes,” The Journal of Nutritional Biochemistry, vol. 25, no. 2, pp. 126–135, 2014. View at: Publisher Site | Google Scholar
  15. C. Tsai, Y. Hsu, H. Ting, C. Huang, and C. Yen, “The in vivo antioxidant and antifibrotic properties of green tea (Camellia sinensis, Theaceae),” Food Chemistry, vol. 136, no. 3-4, pp. 1337–1344, 2013. View at: Publisher Site | Google Scholar
  16. P. Bhardwaj and D. Khanna, “Green tea catechins: defensive role in cardiovascular disorders,” Chinese Journal of Natural Medicines, vol. 11, no. 4, pp. 345–353, 2013. View at: Publisher Site | Google Scholar
  17. M. A. Islam, “Cardiovascular effects of green tea catechins: Progress and promise,” Recent Patents on Cardiovascular Drug Discovery, vol. 7, no. 2, pp. 88–99, 2012. View at: Publisher Site | Google Scholar
  18. G. Grandl and C. Wolfrum, “Hemostasis, endothelial stress, inflammation, and the metabolic syndrome,” Seminars in Immunopathology, vol. 40, no. 2, pp. 215–224, 2018. View at: Publisher Site | Google Scholar
  19. J. Iqbal, A. Al Qarni, A. Hawwari, A. . Alghanem, and A. Gasmelseed, “Metabolic syndrome, dyslipidemia and regulation of lipoprotein metabolism,” Current Diabetes Reviews, vol. 13, 2017. View at: Publisher Site | Google Scholar
  20. Q. Fu, Q. Li, X. Lin et al., “Antidiabetic Effects of Tea,” Molecules, vol. 22, no. 5, p. 849, 2017. View at: Publisher Site | Google Scholar
  21. K. M. Munir, S. Chandrasekaran, F. Gao, and M. J. Quon, “Mechanisms for food polyphenols to ameliorate insulin resistance and endothelial dysfunction: therapeutic implications for diabetes and its cardiovascular complications,” American Journal of Physiology-Endocrinology and Metabolism, vol. 305, no. 6, pp. E679–E686, 2013. View at: Publisher Site | Google Scholar
  22. J. Huang, Y. Wang, Z. Xie, Y. Zhou, Y. Zhang, and X. Wan, “The anti-obesity effects of green tea in human intervention and basic molecular studies,” European Journal of Clinical Nutrition, vol. 68, no. 10, pp. 1075–1087, 2014. View at: Publisher Site | Google Scholar
  23. N. Siriwardhana, N. S. Kalupahana, M. Cekanova, M. LeMieux, B. Greer, and N. Moustaid-Moussa, “Modulation of adipose tissue inflammation by bioactive food compounds,” The Journal of Nutritional Biochemistry, vol. 24, no. 4, pp. 613–623, 2013. View at: Publisher Site | Google Scholar
  24. T. Suzuki, M. Pervin, S. Goto, M. Isemura, and Y. Nakamura, “Beneficial effects of tea and the green tea catechin epigallocatechin-3-gallate on obesity,” Molecules, vol. 21, no. 10, 2016. View at: Google Scholar
  25. A. Faria, D. Pestana, D. Teixeira et al., “Insights into the putative catechin and epicatechin transport across blood-brain barrier,” Food & Function, vol. 2, no. 1, pp. 39–44, 2011. View at: Publisher Site | Google Scholar
  26. I. Figueira, G. Garcia, R. C. Pimpão et al., “Polyphenols journey through blood-brain barrier towards neuronal protection,” Scientific Reports, vol. 7, no. 1, article 11456, 2017. View at: Publisher Site | Google Scholar
  27. E. Mancini, C. Beglinger, J. Drewe, D. Zanchi, U. E. Lang, and S. Borgwardt, “Green tea effects on cognition, mood and human brain function: A systematic review,” Phytomedicine, vol. 34, pp. 26–37, 2017. View at: Publisher Site | Google Scholar
  28. A. Scholey, L. A. Downey, and J. Ciorciari, “Acute neurocognitive effects of epigallocatechin gallate (EGCG),” Appetite, vol. 58, no. 2, pp. 767–770, 2012. View at: Publisher Site | Google Scholar
  29. D. Vauzour, “Dietary polyphenols as modulators of brain functions: biological actions and molecular mechanisms underpinning their beneficial effects,” Oxidative Medicine and Cellular Longevity, vol. 2012, Article ID 914273, 16 pages, 2012. View at: Publisher Site | Google Scholar
  30. A. Araghizadeh, J. Kohanteb, and M. M. Fani, “Inhibitory activity of green tea (Camellia sinensis) extract on some clinically isolated cariogenic and periodontopathic bacteria,” Medical Principles and Practice, vol. 22, no. 4, pp. 368–372, 2013. View at: Publisher Site | Google Scholar
  31. B. U. Aylikci and H. Çolak, “Halitosis: from diagnosis to management,” Journal of Natural Science, Biology and Medicine, vol. 4, no. 1, pp. 14–23, 2013. View at: Publisher Site | Google Scholar
  32. M. D. R. De Moraes, J. R. M. Carneiro, V. F. Passos, and S. L. Santiago, “Effect of green tea as a protective measure against dental erosion in coronary dentine,” Brazilian Oral Research, vol. 30, no. 1, 2016. View at: Publisher Site | Google Scholar
  33. M. Kushiyama, Y. Shimazaki, M. Murakami, and Y. Yamashita, “Relationship between intake of green tea and periodontal disease,” Journal of Periodontology, vol. 80, no. 3, pp. 372–377, 2009. View at: Publisher Site | Google Scholar
  34. B. Narotzki, Y. Levy, D. Aizenbud, and A. Z. Reznick, “Green tea and its major polyphenol EGCG increase the activity of oral peroxidases,” Advances in Experimental Medicine and Biology, vol. 756, pp. 99–104, 2013. View at: Publisher Site | Google Scholar
  35. J. Burana-osot and W. Yanpaisan, “Catechins and caffeine contents of green tea commercialized in Thailand,” Journal of Pharmaceutical and Biomedical Sciences, vol. 22, no. 17, pp. 1–7, 2012. View at: Google Scholar
  36. F. Hajiaghaalipour, J. Sanusi, and M. S. Kanthimathi, “Temperature and Time of Steeping Affect the Antioxidant Properties of White, Green, and Black Tea Infusions,” Journal of Food Science, vol. 81, no. 1, pp. H246–H254, 2016. View at: Publisher Site | Google Scholar
  37. W.-Y. Han, J.-G. Huang, X. Li et al., “Altitudinal effects on the quality of green tea in east China: a climate change perspective,” European Food Research and Technology, vol. 243, no. 2, pp. 323–330, 2017. View at: Publisher Site | Google Scholar
  38. Z.-X. Han, M. M. Rana, G.-F. Liu et al., “Green tea flavour determinants and their changes over manufacturing processes,” Food Chemistry, vol. 212, pp. 739–748, 2016. View at: Publisher Site | Google Scholar
  39. C. Lantano, M. Rinaldi, A. Cavazza, D. Barbanti, and C. Corradini, “Effects of alternative steeping methods on composition, antioxidant property and colour of green, black and oolong tea infusions,” Journal of Food Science and Technology, vol. 52, no. 12, pp. 8276–8283, 2015. View at: Publisher Site | Google Scholar
  40. J.-E. Lee, B.-J. Lee, J.-O. Chung et al., “Geographical and Climatic Dependencies of Green Tea (Camellia sinensis) Metabolites: A 1H NMR-Based Metabolomics Study,” Journal of Agricultural and Food Chemistry, vol. 58, no. 19, pp. 10582–10589, 2010. View at: Publisher Site | Google Scholar
  41. L.-S. Lee, S.-H. Kim, Y.-B. Kim, and Y.-C. Kim, “Quantitative analysis of major constituents in green tea with different plucking periods and their antioxidant activity,” Molecules, vol. 19, no. 7, pp. 9173–9186, 2014. View at: Publisher Site | Google Scholar
  42. M. Liu, H. Tian, J. Wu et al., “Erratum: Relationship between gene expression and the accumulation of catechin during spring and autumn in tea plants (Camellia sinensis L.),” Horticulture Research, vol. 2, no. 1, 2015. View at: Publisher Site | Google Scholar
  43. M. McAlpine and W. Ward, “Influence of Steep Time on Polyphenol Content and Antioxidant Capacity of Black, Green, Rooibos, and Herbal Teas,” Beverages, vol. 2, no. 3, p. 17, 2016. View at: Publisher Site | Google Scholar
  44. S. Sabhapondit, T. Karak, L. P. Bhuyan, B. C. Goswami, and M. Hazarika, “Diversity of catechin in Northeast Indian Tea cultivars,” The Scientific World Journal, vol. 2012, Article ID 485193, 2012. View at: Publisher Site | Google Scholar
  45. S. Saklar, E. Ertas, I. S. Ozdemir, and B. Karadeniz, “Effects of different brewing conditions on catechin content and sensory acceptance in Turkish green tea infusions,” Journal of Food Science and Technology, vol. 52, no. 10, pp. 6639–6646, 2015. View at: Publisher Site | Google Scholar
  46. H. Ashihara, W.-W. Deng, W. Mullen, and A. Crozier, “Distribution and biosynthesis of flavan-3-ols in Camellia sinensis seedlings and expression of genes encoding biosynthetic enzymes,” Phytochemistry, vol. 71, no. 5-6, pp. 559–566, 2010. View at: Publisher Site | Google Scholar
  47. T. Atomssa and A. V. Cholap, “Characterization and determination of catechins in green tea leaves using UV-visible spectrometer,” Journal of Engineering and Technology Research, vol. 7, no. 1, pp. 22–31, 2015. View at: Google Scholar
  48. K. A. Clarke, T. P. Dew, R. E. B. Watson et al., “High performance liquid chromatography tandem mass spectrometry dual extraction method for identification of green tea catechin metabolites excreted in human urine,” Journal of Chromatography B, vol. 972, pp. 29–37, 2014. View at: Publisher Site | Google Scholar
  49. S. Saha, W. Hollands, P. W. Needs et al., “Human O-sulfated metabolites of (-)-epicatechin and methyl-(-)-epicatechin are poor substrates for commercial aryl-sulfatases: Implications for studies concerned with quantifying epicatechin bioavailability,” Pharmacological Research, vol. 65, no. 6, pp. 592–602, 2012. View at: Publisher Site | Google Scholar
  50. A. Zinellu, S. Sotgia, B. Scanu et al., “Human serum albumin increases the stability of green tea catechins in aqueous physiological conditions,” PLoS ONE, vol. 10, no. 7, Article ID e0134690, 2015. View at: Publisher Site | Google Scholar
  51. M. N. Clifford, J. J. van der Hooft, and A. Crozier, “Human studies on the absorption, distribution, metabolism, and excretion of tea polyphenols,” American Journal of Clinical Nutrition, vol. 98, no. 6, pp. 1619S–1630S, 2013. View at: Publisher Site | Google Scholar
  52. M. Renouf, C. Marmet, P. A. Guy et al., “Dose-response plasma appearance of green tea catechins in adults,” Molecular Nutrition & Food Research, vol. 57, no. 5, pp. 833–839, 2013. View at: Publisher Site | Google Scholar
  53. W. C. Reygaert, “The antimicrobial possibilities of green tea,” Frontiers in Microbiology, vol. 5, article 434, 2014. View at: Publisher Site | Google Scholar
  54. H. H. Chow, Y. Cai, D. S. Alberts et al., “Phase I pharmacokinetic study of tea polyphenols following single-dose administration of epigallocatechin gallate and polyphenon E,” Cancer Epidemiology, Biomarkers & Prevention, vol. 10, no. 1, pp. 53–58, 2001. View at: Google Scholar
  55. H.-H. S. Chow, Y. Cai, I. A. Hakim et al., “Pharmacokinetics and safety of green tea polyphenols after multiple-dose administration of epigallocatechin gallate and polyphenon E in healthy individuals,” Clinical Cancer Research, vol. 9, no. 9, pp. 3312–3319, 2003. View at: Google Scholar
  56. M. J. Lee, Z. Y. Wang, and H. Li, “Analysis of plasma and urinary tea polyphenols in human subjects,” Cancer Epidemiology, Biomarkers & Prevention, vol. 4, no. 4, pp. 393–399, 1995. View at: Google Scholar
  57. M. J. Lee, P. Maliakal, L. Chen et al., “Pharmacokinetics of tea catechins after ingestion of green tea and (-)-epigallocatechin-3-gallate by humans: formation of different metabolites and individual variability,” Cancer Epidemiology, Biomarkers & Prevention, vol. 11, no. 10, pp. 1025–1032, 2002. View at: Google Scholar
  58. K. Nakagawa, S. Okuda, and T. Miyazawa, “Dose-dependent incorporation of tea catechins, (-)-epigallocatechin-3-gallate and (-)-epigallocatechin, into human plasma,” Bioscience, Biotechnology, and Biochemistry, vol. 61, no. 12, pp. 1981–1985, 1997. View at: Publisher Site | Google Scholar
  59. U. Ullmann, J. Haller, J. P. Decourt et al., “A single ascending dose study of epigallocatechin gallate in healthy volunteers,” Journal of International Medical Research, vol. 31, no. 2, pp. 88–101, 2003. View at: Publisher Site | Google Scholar
  60. U. Ullmann, J. Haller, J. D. Decourt, J. Girault, V. Spitzer, and P. Weber, “Plasma-kinetic characteristics of purified and isolated green tea catechin epigallocatechin gallate (EGCG) after 10 days repeated dosing in healthy volunteers,” International Journal for Vitamin and Nutrition Research, vol. 74, no. 4, pp. 269–278, 2004. View at: Publisher Site | Google Scholar
  61. C. S. Yang, L. Chen, M. J. Lee, D. Balentine, M. C. Kuo, and S. P. Schantz, “Blood and urine levels of tea catechins after ingestion of different amounts of green tea by human volunteers,” Cancer Epidemiology Biomarkers & Prevention, vol. 7, no. 4, pp. 351–354, 1998. View at: Google Scholar
  62. L. E. Rhodes, G. Darby, K. A. Massey et al., “Oral green tea catechin metabolites are incorporated into human skin and protect against UV radiation-induced cutaneous inflammation in association with reduced production of pro-inflammatory eicosanoid 12-hydroxyeicosatetraenoic acid,” British Journal of Nutrition, vol. 110, no. 5, pp. 891–900, 2013. View at: Publisher Site | Google Scholar
  63. J. Jeon, J. H. Kim, and C. K. Lee, “The antimicrobial activity of (-)-epigallocatechin-3-gallate and green tea extracts against Pseudomonas aeruginosa and Escherichia coli isolated from skin wounds,” Annals of Dermatology, vol. 26, no. 5, pp. 564–569, 2014. View at: Publisher Site | Google Scholar
  64. J. Steinmann, J. Buer, T. Pietschmann, and E. Steinmann, “Anti-infective properties of epigallocatechin-3-gallate (EGCG), a component of green tea,” British Journal of Pharmacology, vol. 168, no. 5, pp. 1059–1073, 2013. View at: Publisher Site | Google Scholar
  65. M. Nakayama, K. Shimatani, T. Ozawa et al., “Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis,” Bioscience, Biotechnology, and Biochemistry, vol. 79, no. 5, pp. 845–854, 2015. View at: Publisher Site | Google Scholar
  66. A. Ben Lagha, B. Haas, and D. Grenier, “Tea polyphenols inhibit the growth and virulence properties of Fusobacterium nucleatum,” Scientific Reports, vol. 7, 2017. View at: Google Scholar
  67. K.-M. Lee, M. Yeo, J.-S. Choue et al., “Protective mechanism of epigallocatechin-3-gallate against Helicobocter pylori-induced gastric epithelial cytotoxicity via the blockage of TLR-4 signaling,” Helicobacter, vol. 9, no. 6, pp. 632–642, 2004. View at: Publisher Site | Google Scholar
  68. X. Xu, X. D. Zhou, and C. D. Wu, “The tea catechin epigallocatechin gallate suppresses cariogenic virulence factors of Streptococcus mutans,” Antimicrobial Agents and Chemotherapy, vol. 55, no. 3, pp. 1229–1236, 2011. View at: Publisher Site | Google Scholar
  69. R. Kanagaratnam, R. Sheikh, F. Alharbi, and D. H. Kwon, “An efflux pump (MexAB-OprM) of Pseudomonas aeruginosa is associated with antibacterial activity of Epigallocatechin-3-gallate (EGCG),” Phytomedicine, vol. 36, pp. 194–200, 2017. View at: Publisher Site | Google Scholar
  70. S. Lee, G. S. A. Razqan, and D. H. Kwon, “Antibacterial activity of epigallocatechin-3-gallate (EGCG) and its synergism with β-lactam antibiotics sensitizing carbapenem-associated multidrug resistant clinical isolates of Acinetobacter baumannii,” Phytomedicine, vol. 24, pp. 49–55, 2017. View at: Publisher Site | Google Scholar
  71. H. Gradišar, P. Pristovšek, A. Plaper, and R. Jerala, “Green tea catechins inhibit bacterial DNA gyrase by interaction with its ATP binding site,” Journal of Medicinal Chemistry, vol. 50, no. 2, pp. 264–271, 2007. View at: Publisher Site | Google Scholar
  72. C. Kohda, Y. Yanagawa, and T. Shimamura, “Epigallocatechin gallate inhibits intracellular survival of Listeria monocytogenes in macrophages,” Biochemical and Biophysical Research Communications, vol. 365, no. 2, pp. 310–315, 2008. View at: Publisher Site | Google Scholar
  73. Y. Wang and S. Ma, “Recent advances in inhibitors of bacterial fatty acid synthesis type II (FASII) system enzymes as potential antibacterial agents,” ChemMedChem, vol. 8, no. 10, pp. 1589–1608, 2013. View at: Publisher Site | Google Scholar
  74. L. G. Xiong, Y. J. Chen, J. W. Tong et al., “Tea polyphenol epigallocatechin gallate inhibits Escherichia coli by increasing endogenous oxidative stress,” Food Chemistry, vol. 217, pp. 196–204, 2017. View at: Publisher Site | Google Scholar
  75. Y. S. Cho, N. L. Schiller, H. Y. Kahng, and K. H. Oh, “Cellular responses and proteomic analysis of Escherichia coli exposed to green tea polyphenols,” Current Microbiology, vol. 55, no. 6, pp. 501–506, 2007. View at: Publisher Site | Google Scholar
  76. T. W. Sirk, E. F. Brown, M. Friedman, and A. K. Sum, “Molecular binding of catechins to biomembranes: relationship to biological activity,” Journal of Agricultural and Food Chemistry, vol. 57, no. 15, pp. 6720–6728, 2009. View at: Publisher Site | Google Scholar
  77. T. W. Sirk, E. F. Brown, A. K. Sum, and M. Friedman, “Molecular dynamics study on the biophysical interactions of seven green tea catechins with lipid bilayers of cell membranes,” Journal of Agricultural and Food Chemistry, vol. 56, no. 17, pp. 7750–7758, 2008. View at: Publisher Site | Google Scholar
  78. O. Levinger, T. Bikels-Goshen, E. Landau, M. Fichman, and R. Shapira, “Epigallocatechin gallate induces upregulation of the two-component VraSR system by evoking a cell wall stress response in Staphylococcus aureus,” Applied and Environmental Microbiology, vol. 78, no. 22, pp. 7954–7959, 2012. View at: Publisher Site | Google Scholar
  79. P. D. Stapleton, S. Shah, K. Ehlert, Y. Hara, and P. W. Taylor, “The β-lactam-resistance modifier (-)-epicatechin gallate alters the architecture of the cell wall of Staphylococcus aureas,” Microbiology, vol. 153, no. 7, pp. 2093–2103, 2007. View at: Publisher Site | Google Scholar
  80. A. Sharma, S. Gupta, I. P. Sarethy, S. Dang, and R. Gabrani, “Green tea extract: Possible mechanism and antibacterial activity on skin pathogens,” Food Chemistry, vol. 135, no. 2, pp. 672–675, 2012. View at: Publisher Site | Google Scholar
  81. M. Stenvang, M. S. Dueholm, B. S. Vad et al., “Epigallocatechin gallate remodels overexpressed functional amyloids in pseudomonas aeruginosa and increases biofilm susceptibility to antibiotic treatment,” The Journal of Biological Chemistry, vol. 291, no. 51, pp. 26540–26553, 2016. View at: Publisher Site | Google Scholar
  82. M. Spina, M. Cuccioloni, M. Mozzicafreddo et al., “Mechanism of inhibition of wt-dihydrofolate reductase from E. coli by tea epigallocatechin-gallate,” Proteins: Structure, Function, and Genetics, vol. 72, no. 1, pp. 240–251, 2008. View at: Publisher Site | Google Scholar
  83. N. Chinnam, P. K. Dadi, S. A. Sabri, M. Ahmad, M. A. Kabir, and Z. Ahmad, “Dietary bioflavonoids inhibit Escherichia coli ATP synthase in a differential manner,” International Journal of Biological Macromolecules, vol. 46, no. 5, pp. 478–486, 2010. View at: Publisher Site | Google Scholar
  84. W.-H. Zhao, Z.-Q. Hu, Y. Hara, and T. Shimamura, “Inhibition by epigallocatechin gallate (EGCg) of conjugative R plasmid transfer in Escherichia coli,” Journal of Infection and Chemotherapy, vol. 7, no. 3, pp. 195–197, 2001. View at: Publisher Site | Google Scholar
  85. E. Aboulmagd, H. I. Al-Mohamme, and S. Al-Badry, “Synergism and Postantibiotic Effect of Green Tea Extract and Imipenem Against Methicillin-resistant Staphylococcus aureus,” Journal of Microbiology, vol. 1, no. 3, pp. 89–96, 2011. View at: Publisher Site | Google Scholar
  86. J. Fournier-Larente, M.-P. Morin, and D. Grenier, “Green tea catechins potentiate the effect of antibiotics and modulate adherence and gene expression in Porphyromonas gingivalis,” Archives of Oral Biolog, vol. 65, pp. 35–43, 2016. View at: Publisher Site | Google Scholar
  87. B. Haghjoo, L. H. Lee, U. Habiba, H. Tahir, M. Olabi, and T. Chu, “The synergistic effects of green tea polyphenols and antibiotics against potential pathogens,” Advances in Bioscience and Biotechnology, vol. 04, no. 11, pp. 959–967, 2013. View at: Publisher Site | Google Scholar
  88. A. Noormandi and F. Dabaghzadeh, “Effects of green tea on Escherichia coli as a uropathogen,” Journal of Traditional and Complementary Medicine, vol. 5, no. 1, pp. 15–20, 2015. View at: Publisher Site | Google Scholar
  89. D. N. Passat, “Interactions of Black and Green Tea Water Extracts with Antibiotics Activity in Local Urinary Isolated Escherichia coli,” Journal of Al-Nahrain University Science, vol. 15, no. 3, pp. 134–142, 2012. View at: Publisher Site | Google Scholar
  90. R. C. Fink, B. Roschek Jr., and R. S. Alberte, “HIV type-1 entry inhibitors with a new mode of action,” Antiviral Chemistry & Chemotherapy, vol. 19, no. 6, pp. 243–255, 2009. View at: Publisher Site | Google Scholar
  91. I. Hauber, H. Hohenberg, B. Holstermann, W. Hunstein, and J. Hauber, “The main green tea polyphenol epigallocatechin-3-gallate counteracts semen-mediated enhancement of HIV infection,” Proceedings of the National Acadamy of Sciences of the United States of America, vol. 106, no. 22, pp. 9033–9038, 2009. View at: Publisher Site | Google Scholar
  92. Y. Lin, Y. Wu, C. Tseng et al., “Green Tea Phenolic Epicatechins Inhibit Hepatitis C Virus Replication via Cycloxygenase-2 and Attenuate Virus-Induced Inflammation,” PLoS ONE, vol. 8, no. 1, p. e54466, 2013. View at: Publisher Site | Google Scholar
  93. S. Liu, H. Li, L. Chen et al., “(-)-epigallocatechin-3-gallate inhibition of epstein-barr virus spontaneous lytic infection involves ERK1/2 and PI3-K/Akt signaling in EBV-positive cells,” Carcinogenesis, vol. 34, no. 3, pp. 627–637, 2013. View at: Publisher Site | Google Scholar
  94. J.-Y. Pang, K.-J. Zhao, J.-B. Wang, Z.-J. Ma, and X.-H. Xiao, “Green tea polyphenol, epigallocatechin-3-gallate, possesses the antiviral activity necessary to fight against the hepatitis B virus replication in vitro,” Journal of Zhejiang University SCIENCE B, vol. 15, no. 6, pp. 533–539, 2014. View at: Publisher Site | Google Scholar
  95. J. Yang, L. Li, S. Tan et al., “A natural theaflavins preparation inhibits HIV-1 infection by targeting the entry step: Potential applications for preventing HIV-1 infection,” Fitoterapia, vol. 83, no. 2, pp. 348–355, 2012. View at: Publisher Site | Google Scholar
  96. J. Xu, Z. Xu, W. Zheng et al., “A review of the antiviral role of green tea catechins,” Molecules, vol. 22, no. 8, Article ID 1337, 2017. View at: Publisher Site | Google Scholar
  97. K. Matsumoto, H. Yamada, N. Takuma, H. Niino, and Y. M. Sagesaka, “Effects of green tea catechins and theanine on preventing influenza infection among healthcare workers: a randomized controlled trial,” BMC Complementary and Alternative Medicine, vol. 11, no. 15, 2011. View at: Publisher Site | Google Scholar
  98. M. Aboulaila, N. Yokoyama, and I. Igarashi, “Inhibitory effects of (-)-Epigallocatechin-3-gallate from green tea on the growth of Babesia parasites,” Parasitology, vol. 137, no. 5, pp. 785–791, 2010. View at: Publisher Site | Google Scholar
  99. M. C. Güida, M. I. Esteva, A. Camino, M. M. Flawiá, H. N. Torres, and C. Paveto, “Trypanosoma cruzi: in vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg),” Experimental Parasitology emphasizes, vol. 117, no. 2, pp. 188–194, 2007. View at: Publisher Site | Google Scholar
  100. J. D. F. Inacio, L. Gervazoni, M. M. Canto-Cavalheiro, and E. E. Almeida-Amaral, “The Effect of (-)-Epigallocatechin 3-O - Gallate In Vitro and In Vivo in Leishmania braziliensis: Involvement of Reactive Oxygen Species as a Mechanism of Action,” PLOS Neglected Tropical Diseases, vol. 8, no. 8, Article ID e3093, 2014. View at: Publisher Site | Google Scholar
  101. P. Thipubon, C. Uthaipibull, S. Kamchonwongpaisan, W. Tipsuwan, and S. Srichairatanakool, “Inhibitory effect of novel iron chelator, 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract on growth of Plasmodium falciparum,” Malaria Journal, vol. 14, no. 1, article no. 382, 2015. View at: Publisher Site | Google Scholar
  102. P. A. Vigueira, S. S. Ray, B. A. Martin, M. M. Ligon, and K. S. Paul, “Effects of the green tea catechin (-)-epigallocatechin gallate on Trypanosoma brucei,” International Journal for Parasitology: Drugs and Drug Resistance, vol. 2, pp. 225–229, 2012. View at: Publisher Site | Google Scholar
  103. C. R. Da Silva, J. B. De Andrade Neto, R. De Sousa Campos et al., “Synergistic effect of the flavonoid catechin, quercetin, or epigallocatechin gallate with fluconazole induces apoptosis in Candida tropicalis resistant to fluconazole,” Antimicrobial Agents and Chemotherapy, vol. 58, no. 3, pp. 1468–1478, 2014. View at: Publisher Site | Google Scholar
  104. M. Muthu, J. Gopal, S. X. Min, and S. Chun, “Green Tea Versus Traditional Korean Teas: Antibacterial/Antifungal or Both?” Applied Biochemistry and Biotechnology, vol. 180, no. 4, pp. 780–790, 2016. View at: Publisher Site | Google Scholar
  105. Y. Ning, J. Ling, and C. D. Wu, “Synergistic effects of tea catechin epigallocatechin gallate and antimycotics against oral Candida species,” Archives of Oral Biolog, vol. 60, no. 10, pp. 1565–1570, 2015. View at: Publisher Site | Google Scholar
  106. B. J. Park, H. Taguchi, K. Kamei, T. Matsuzawa, S.-H. Hyon, and J.-C. Park, “In vitro antifungal activity of epigallocatechin 3-O-gallate against clinical isolates of dermatophytes,” Yonsei Medical Journal, vol. 52, no. 3, pp. 535–538, 2011. View at: Publisher Site | Google Scholar
  107. A. Thomas, S. Thakur, R. Habib, and N. Marwah, “Comparison of Antimicrobial Efficacy of Green Tea, Garlic with Lime, and Sodium Fluoride Mouth Rinses against Streptococcus mutans, Lactobacilli species, and Candida albicans in Children: A Randomized Double-blind Controlled Clinical Trial,” International Journal of Clinical Pediatric Dentistry, vol. 10, no. 3, pp. 234–239, 2017. View at: Publisher Site | Google Scholar
  108. B. E. Roberts, M. L. Duennwald, H. Wang et al., “A synergistic small-molecule combination directly eradicates diverse prion strain structures,” Nature Chemical Biology, vol. 5, no. 12, pp. 936–946, 2009. View at: Publisher Site | Google Scholar
  109. A. Farooqui, A. Khan, I. Borghetto, S. U. Kazmi, S. Rubino, and B. Paglietti, “Synergistic antimicrobial activity of Camellia sinensis and Juglans regia against multidrug-resistant bacteria,” PLoS ONE, vol. 10, no. 2, Article ID e0118431, 2015. View at: Publisher Site | Google Scholar
  110. C. A. Rowe, M. P. Nantz, J. F. Bukowski, and S. S. Percival, “Specific formulation of Camellia sinensis prevents cold and flu symptoms and enhances gamma, delta T cell function: a randomized, double-blind, placebo-controlled study,” Journal of the American College of Nutrition, vol. 26, no. 5, pp. 445–452, 2007. View at: Publisher Site | Google Scholar
  111. M. Park, H. Yamada, K. Matsushita et al., “Green tea consumption is inversely associated with the incidence of influenza infection among schoolchildren in a tea plantation area of Japan,” Journal of Nutrition, vol. 141, no. 10, pp. 1862–1870, 2011. View at: Publisher Site | Google Scholar
  112. T. Noda, T. Ojima, S. Hayasaka, C. Murata, and A. Hagihara, “Gargling for oral hygiene and the development of fever in childhood: A population study in Japan,” Journal of Epidemiology, vol. 22, no. 1, pp. 45–49, 2012. View at: Publisher Site | Google Scholar
  113. H. Yamada, N. Takuma, T. Daimon, and Y. Hara, “Gargling with tea catechin extracts for the prevention of influenza infection in elderly nursing home residents: a prospective clinical study,” The Journal of Alternative and Complementary Medicine, vol. 12, no. 7, pp. 669–672, 2006. View at: Publisher Site | Google Scholar
  114. H. Yamada, T. Daimon, K. Matsuda et al., “A randomized controlled study on the effects of gargling with tea catechin extracts on the prevention of influenza in healthy adults,” Japanese Journal of Clinical Pharmacology and Therapeutics, vol. 38, no. 5, pp. 323–330, 2007. View at: Google Scholar

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