Fluorescent Microneutralization Assay

The microneutralization assay is a highly sensitive and specific assay for detecting virus-specific neutralizing antibodies and their corresponding titers that is amenable to high-density microplates. Immunofluorescence staining of a viral protein, or the use of viruses expressing a fluorescent reporter construct, provides a robust assay format for measuring the neutralizing effects of test antibodies. Using the Lionheart™ FX Automated Microscope or Cytation™ Cell Imaging Multi-Mode Reader, microplates containing target cells exposed to viral treatment and increasing concentrations of test neutralizing antibodies are imaged. Processed images are then analyzed to determine the number and fluorescence intensity of viral foci using automated Gen5™image analysis tools.


Microneutralization Assay Procedure


Samples are imaged using automated fluorescence microscopy and then processed to reduce background signal.



Gen5 cellular analysis tools identify viral foci (yellow masks) using size and signal threshold criteria to detect small changes between samples.


Number and intensity of viral foci are automatically calculated and reported for each sample.


Increase assay throughput and performance


Liquid handling automation

The BioTek MultiFlo™ FX or other Liquid Handler can be used to automate tedious fixing and staining steps


BioStack™ Compatible Assay:
Process up to 50 microplates

Incorporating the BioStackMicroplate Stacker enables processing and analysis of up to 50 plates


BioSpa™ Compatible Assay: Live-cell analysis on up to 8 microplates

The BioSpa Live Cell Analysis System
enables multi-plate kinetic analysis of the
label-free TCID50 when integrated with
Cytation Live Cell Imager







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microneutralization assay